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Research Article

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Ana Neacsucorresponding author
Daniela Gheorghe

Abstract

An EPR study of paramagnetic centers formed by irradiation of polycrystalline L-α-anhydrous asparagine (L-Asn) was performed. The EPR spectra of gamma irradiated samples at room temperature, shown the presence of three types of paramagnetic centers. A possible mechanisms of formation for the three radical species is suggested, based also on literature data. The kinetics of the disappearance of radicals during thermal annealing indicated a complex mechanism.

Keywords
L-α- anhydrous asparagine, ionizing radiation, paramagnetic centers, thermal disappearance, unpaired electron

Article Details

How to Cite
Neacsu, A., & Gheorghe, D. (2019). Kinetics of the thermal disappearance of radicals formed during the radiolysis of L-α- anhydrous asparagine. Chemical Reports, 1(1), 13-21. https://doi.org/10.25082/CR.2019.01.002

References

  1. Lund P. Nitrogen Metabolism in Mammalian, Applied Science, Barking, 1981.
  2. Robinson EN and Robinson BA. Deamidation of human proteins. Proceedings of the National Academy of Sciences, 2001, 98(22): 12409-12414.https://doi.org/10.1073/pnas.221463198
  3. Hipkiss AR. On the “struggle between chemistry and biology during aging”-implications for DNA repair, apoptosis and proteolysis, and a novel route of intervention. Biogerontology, 2001, 2(3): 173-178.https://doi.org/10.1023/A:1011599321168
  4. Bambai B, Rogee CE, Stec B, et al. Role of Asn-382 and Thr-383 in activation and inactivation of human prostaglandin H synthase cyclooxygenase catalysis. The Journal of Biological Chemistry, 2003, 279: 4084-4092.https://doi.org/10.1074/jbc.M304762200
  5. Vijayakumar M, Qian H and Zhou HX. Hydrogen bonds between short polar side chains and peptides backbone: prevalence in proteins and effects on helix-forming propensities. Proteins, 1999, 34: 497-507.https://doi.org/10.1002/(SICI)1097-0134(19990301)34:4<497::AID-PROT9>3.0.CO;2-G
  6. Cooper HJ, Hudgins RR, Hakanson K, et al. Characterization of amino acid side chain losses in electron capture dissociation. Journal of The American Society for Mass Spectrometry, 2002, 13: 241-249.https://doi.org/10.1016/S1044-0305(01)00357-9
  7. Zhang P, Han S, Zhan Y, et al. Neutron spectroscopic and Raman studies of interaction between water and proline. Journal of Chemical Physics, 2008, 345: 196-199.
  8. Box HC, Freund GH, Lilga TK, et al. Hyperfine couplings in primary radiation products. Journal of Chemical Physics, 1975, 63: 2059-2063.https://doi.org/10.1063/1.431543
  9. Strzelczak G, Berges J, Housee-Levin C, et al. EPR spectroscopy and theoretical study of γ-irradiated asparagine and aspartic acid in solid state. Biophysical Chemistry, 2007, 125: 92-103.https://doi.org/10.1016/j.bpc.2006.06.017
  10. Contineanu M. Thermal decay kinetics of the radicals generated during the radiolysis of polyacrylamide (PAA) hydrolized to different extents. Revue Roumaine de Chimie, 1996, 41(9): 703-711.
  11. Contineanu M and Neacşu A. Radical species formed by gamma radiolysis of polycrystalline telluric acid. Revue Roumaine de Chimie, 2006, 51(11):1069-1078.
  12. Contineanu M and Neacşu A. PbCrO4 radiolysis impurified with CrO3. Revista de Chimie (Bucharest), 2006, 57(7): 706-710.
  13. Tria JJ, HoelD and Johnsen HR. The kinetics of radical decay in crystalline amino acids. 7. Monohydrates. Journal of Physical Chemistry, 1979, 83(24): 3174-3179.https://doi.org/10.1021/j100487a022
  14. Waite RT. Theoretical Treatment of the Kinetics of Diffusion-Limited Reactions. Physical Review, 1957, 107(2): 463-475.https://doi.org/10.1103/PhysRev.107.463
  15. Yung Y and Lee S. Equivalence of the radical recombination rate theories of Waite and Szabo. Chemical Physics Letters, 1994, 231: 429-438.https://doi.org/10.1016/0009-2614(94)01282-2
  16. Zubavichus Y, Fuchs O, Weinhardt L, et al. Denlinger J D, Grunze M, Soft X-Ray-Induced Decomposition of Amino Acids: An XPS, Mass Spectrometry, and NEXAFS Study. Radiation Research, 2004, 161(3): 346-358.https://doi.org/10.1667/RR3114.1
  17. Close MD, Fouse WG and Bernhard AW. ESR and ENDOR study of single crystals of L-asparagine-H2O x-irradiated at room temperature. The Journal of Chemical Physics, 1977, 66(4): 1534-1540.https://doi.org/10.1063/1.434117