Open Access Peer-reviewed Research Article

RETRACTED ARTICLE: An Easy Method to Non-Destructively Separate the Strands of a Circular Duplex Plasmid Chromosome: A Preliminary Report

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Submitted   May 30, 2025
Published   Sep 6, 2025
Issue    Vol 5 No 1 (2024)
DOI   10.25082/CR.2024.01.005

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Ken Biegeleisen corresponding author
649 Kissam Road, Peekskill, NY 10566, USA

Abstract

At the request of the author, this article is hereby retracted from Chemical Reports.
Reason for Retraction
1. Methodological Flaw: The reported electrophoretic separation of plasmid pBR322 strands was based on a unique 2-step denaturation procedure. Subsequent investigations revealed that the bands observed were not the top and bottom strands of pBR322, as originally claimed.
2. Critical Oversight: The experimental outcome was irreproducible due to unanticipated RNase A treatment of the DNA samples, which removed the mRNA binding sites essential for the proposed separation mechanism.
3. Research Integrity: To prevent further dissemination of potentially misleading findings, the authors voluntarily requested retraction.
Ethical Compliance
This retraction follows COPE guidelines (Section 4.11) and has been approved by the Editorial Board of Chemical Reports.

Keywords
DNA topology, plasmid, Form IV, strand separation, non-helical DNA, circular DNA

Article Details

How to Cite
Biegeleisen, K. (2025). RETRACTED ARTICLE: An Easy Method to Non-Destructively Separate the Strands of a Circular Duplex Plasmid Chromosome: A Preliminary Report. Chemical Reports, 5(1), 298-307. https://doi.org/10.25082/CR.2024.01.005
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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