Journal of Pharmaceutical and Biopharmaceutical Research (JPBR) (ISSN:2630-533X)  is an open access, continuously published, international, refereed  journal. The aim of the journal is to provide the authors a timely and peer reviewed process for evaluation and publication of their manuscripts. All articles submitted to JPBR will undergo a rigorous double-blind peer review, and all published articles can be downloaded and read for free. JPBR will pay wide attention to the trends in related fields and insist on publishing original research work of highest quality.

JPBR publishes high quality original research work, reviews, and short communications in the following areas:
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--Nanotechnology for Pharmaceutical Drug Formulations
Biopharmaceutical Sciences:
--Molecular Biology
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Vol 3 No 1 (2021)

Published: 2021-06-18

Abstract views: 392   PDF downloads: 223  

Page 169-175

Pharmacokinetics study of potential anti-CML drug Cyclobentinib (CB1107) by HPLC–MS/MS

blankpage Xinghua Zhao, Jiaojiao Zhang, Yutong Liang, Jie Li, Shi Ding, Yang Wang, Ye Chen, Ju Liu

Purpose: A simple, sensitive and specific HPLC–MS/MS method was established to analysis the pharmacokinetics of CB1107 in mouses. Methods: A simple, selective, and sensitive high-throughput liquid chromatography-tandem mass spectrometry (LC-MS-MS) method has been developed and validated for quantitative determination of CB1107 in rat serum.Chromatographic separation was achieved on a Zorbax Extend C18 Rapid Resolution HD column (4.6 mm × 50 mm, 1.8 μm). The column temperature was maintained at 35℃ and at flow rate of 0.6 mL/min. Injection volume was 20 μL. The mobile phases consisted of 0.1% formic acid in water (mobile phase A)and 0.1% formic acid in acetonitrile (mobile phase B), and total run time was 30min. MS-MS detection was performed in the selected monitoring mode of electrospray positive ionization reaction. Results: The pharmacokinetic characteristics of CB1107 in mice belong to the two-compartment model.When the doses were 400 mg/kg, 600 mg/kg and 800 mg/kg, corresponding area under the plasma concentration-time curve (AUC) respectively were 20.011±1.24 mg/h/L, 26.778±2.19 mg/h/L, 38.82±1.44 mg/h/L, suggesting that CB1107 have a good absorption in the body.And the AUC of three doses are proportional, indicating that CB1107 conforms to linear pharmacokinetics in vivo. Conclusion: This method was successfully applied to study the pharmacokinetics at three different doses of CB1107 after oral administration in mouses. In this study, the bioactivity mechanism of CB1107, by the pharmacokinetic investigation of CB1107 in vivo.

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Prof. Pal Perjesi-photo  ISSN: 2630-533X
 Abbreviation: J Pharm Biopharm Res
 Editor-in-Chief: Prof. Pal Perjesi (Hungary)
 Publishing Frequency: Continuous publication
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