Advances in Biochips (AB) (ISSN:2630-4511) is an international peer-reviewed journal to show new ideas and optimize methods of this promising technology. With a broad range of disciplines and expanding topics, this journal encourages submissions of original research, review article, commentary, perspective, opinion, and so on.

Topics of interest include, but are not limited to the following:
--Disease diagnosis
--Detection of bio agents
--DNA microarray
--Protein array
--Cell chip
--Tissue microarray
--Sequencing technology
--Chemical compound microarray

Vol 1 No 1 (2019)

Published: 2019-02-27

Abstract views: 697   PDF downloads: 325   HTML downloads: 266  

Page 2-11

Biosensor for maltose quantification and estimation of maltase activity

blankpage Elena V. Emelyanova

The aim of this study was to create a laboratory model of an amperometric microbial biosensor for maltose quantification in the presence and absence of starch and to estimate the use of the model in the study of maltase activity of the culture-receptor. The biosensor for maltose was developed on the basis of a Clark-type oxygen electrode, coupled with a bioreceptor, which contained bacterial cells immobilized on the membrane. The determination of maltose concentration was based on measuring the rate of electrode current change in response to addition of the analyte. The detection limit of the biosensor was 1 µM maltose, a linear interval of standard curve was observed from 14 µM up to 1.9 mM of maltose. The microbial biosensor demonstrated good sensitivity to maltose, 36.02 nА (M·s)-1. Combination of bioreceptors on the basis of fungus and bacterium allowed of using the biosensor for quantification of maltose in the presence of starch. Changes in metabolism of the culture-receptor had an effect on the biosensor response. It indicated that the developed model was a tool of simple construction and easy-to-use in the study of maltase activity of the immobilized culture-receptor.

Abstract views: 799   PDF downloads: 395   HTML downloads: 184  

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Zhenqing Li-photo  ISSN: 2630-4511
 Editor-in-Chief: Dr. Zhenqing Li(China)
 Publishing Frequency: Bi-annual
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 Publishing Model:
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