Open Access Peer-reviewed Research Article

Effect of experimental hyperglycemia on intestinal elimination and biliary excretion of ibuprofen enantiomers in hyperglycemic rats

Main Article Content

Hawsar Othman Mohamed
Attila Almási
Pal Perjesi corresponding author


Diabetic complications are mostly due to hyperglycemia. Hyperglycemia is reported to be associated with oxidative stress. It can result in changes in the activities of drug-metabolizing enzymes and membrane-integrated transporters, which can modify the fate of drugs and other xenobiotics. An in vivo intestinal perfusion model was used to investigate how experimental hyperglycemia affects intestinal elimination and biliary excretion of ibuprofen enantiomers in the rat. Experimental diabetes was induced by intravenous (i.v.) administration of streptozotocin. The intestinal perfusion medium contained 250 µM racemic ibuprofen. A validated isocratic chiral HPLC method with UV detection was developed to determine the amount of the two enantiomers in the intestinal perfusate and the bile. The results indicated that experimental diabetes doesn’t cause a statistically significant difference in the disappearance of ibuprofen enantiomers from the small intestine. Analysis of the bile samples detected only the (S)-IBP enantiomer. Excretion of the ibuprofen enantiomer to the bile decreased in experimental diabetes. The observed changes can affect the pharmacokinetics of drugs administered in hyperglycemic individuals.

streptozotocin, hyperglycaemia, (S)-ibuprofen, biliary excretion

Article Details

Author Biography

Pal Perjesi, Institute of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Pécs, Pécs, Hungary

The editor-in-chief of Journal of Pharmaceutical and Biopharmaceutical Research.

Supporting Agencies
This study was supported by the European Union and co-financed by the European Social Fund (EFOP-3.6.1.-16-2016-00004). The financial support is highly appreciated.
How to Cite
Mohamed, H. O., Almási, A., & Perjesi, P. (2022). Effect of experimental hyperglycemia on intestinal elimination and biliary excretion of ibuprofen enantiomers in hyperglycemic rats. Journal of Pharmaceutical and Biopharmaceutical Research, 4(2), 283-295.


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