Open Access Peer-reviewed Research Article

Main Article Content

Qingfeng Meng
Lunwei Zhu
Yao Zhang
Longjie Zhang
Shaobin Fu corresponding author

Abstract

Microorganisms causing the phenomenon of Shenghua were isolated, purified and identified from fermented chili pepper by potato glucose media (PDA) and nutrient agar media (NA) via streak plate method. The purified strains were validated by tie-back test and identified by phenotype and genotype methods. Results showed that two strains were isolated from the fermented chili with Shenghua. It was identified as Pichia kudriavzevii that causing Shenghua by morphological and molecular biology from PDA media while the strain isolated from the NA media that could not cause Shenghua was identified as gram-positive bacterium by microscopic and colony morphology.

Keywords
fermented chilli pepper, ‘Shenghua’, Pichia kudriavzevii, Guizhou

Article Details

Supporting Agencies
This research was financially supported by Training Programs of Innovation and Entrepreneurship for Undergraduates (ZKDC 2018016, ZYDC2018117).
How to Cite
Meng, Q., Zhu, L., Zhang, Y., Zhang, L., & Fu, S. (2019). Isolation and identification of ‘Shenghua’ microorganisms in fermented chili pepper from Southwest China. Journal of Pharmaceutical and Biopharmaceutical Research, 1(2), 70-73. https://doi.org/10.25082/JPBR.2019.02.004

References

  1. Hu Y. Interpretation of the Name of Pepper. Ancient & Modern Agriculture, 2013, 4: 67-75.
  2. HervertHern´andez D. Bioactive compounds of four hot pepper varieties (Capsicum annuum L.), antioxidant capacity, and intestinal bioaccessibility. Journal of Agricultural & Food Chemistry, 2010, 58(6): 3399-3406. https://doi.org/10.1021/jf904220w
  3. Maji AK and Banerji P. Phytochemistry and gastrointestinal benefits of the medicinal spice, Capsicum annuum L. (Chilli): a review. Journal of Complementary and Integrative Medicine, 2016, 13(2): 97-122. https://doi.org/10.1515/jcim-2015-0037
  4. Pino J, Maril´u Gonz´alez, Ceballos L, et al. Characterization of total capsaicinoids, colour and volatile compounds of Habanero chilli pepper (Capsicum chinense Jack.) cultivars grown in Yucatan. Food Chemistry, 2007, 104(4): 1682- 1686. https://doi.org/10.1016/j.foodchem.2006.12.067
  5. Yan Y. Determination of Vitamin C in Food. Popular Standardization, 2015. 11: 67-69.
  6. Tan CK, Ali ZM and Zainal Z. Changes in ethylene production, carbohydrase activity and antioxidant status in pepper fruits during ripening. Scientia Horticulturae, 2012, 142(4): 23-31. https://doi.org/10.1016/j.scienta.2012.04.030
  7. Zhang L. Effect of Capsaicin on Cholesterol Reduction in Rats and Its Molecular Mechanisms. 2013, Southwest University: Chongqing.
  8. Zhang Z, Jiang Y and Tian L. Studies on stabilities of capsicum pigment and application in food. china Condiment, 2006, 4(21): 30-35.
  9. Shi Q. Study on the Development of Pepper Industry in Zunyi City, Guizhou Province. Rural Economy and Science- Technology, 2017, 28(8): 152-153.
  10. Guo L. Regulatory effect of lactic acid bacteria on intestinal flora disturbalance in mice. 2017, Yangzhou University: Yangzhou.
  11. Lin AX, Min CY, Jiao X, et al. Isolation and Identification of Spoilage Microorganisms from Sichuan Pickles. Food Science, 2013, 34(21): 204-208.
  12. Li H, Xiao Q, Liu N, et al. Analysis of Edible Fungi Degraded Reasons and Discussion of Rejuvenation Method. Liaoning Agricultural Sciences, 2010, 30(5): 53-55. https://doi.org/10.1080/00949651003724790